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Objective@#To study the effect of puerarin injection on the antioxidant capacity of patients with viral myocarditis (VMC).@*Methods@#Seventy-four patients with VMC who were admitted during the period from January 2014 to March 2017 were randomly divided into the observation group and the control group, 37 cases in each group. The control group was given routine treatment, and on this basis, the observation group was additionally treated with intravenous infusion of puerarin injection. The curative effect and safety were evaluated after 14 days of intervention. Myocardial enzymes [creatine kinase isoenzyme (CK-MB), troponin T (cTnT)], inflammation [high-sensitivity C-reactive protein (hs-CRP), interleukin-35 (IL-35)] and antioxidant indexes [total antioxidant capacity (T-AOC), superoxide dismutase (SOD), reduced glutathione (GSH)] were determined before and after treatment.@*Results@#The total treatment response rate of the observation group was significantly higher than that of the control group (94.59% vs 72.97%) (χ2=6.366, P=0.012). The levels of serum CK-MB, cTnT and hs-CRP in the observation group were significantly lower than those in the control group after treatment (t=8.279, 21.907, 9.703, all P=0.000), while levels of IL-35, T-AOC, SOD and GSH were significantly higher than those in the control group (t=10.194, 11.128, 15.539, 11.390, all P=0.000). There were 2 cases of mild fever in the observation group and 1 case of transient abdominal distension.@*Conclusions@#Puerarin injection is safe and effective in the treatment of VMC. It can significantly inhibit inflammation and improve the antioxidant capacity.
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Objective To observe the influence of Simvastatin on the ATP-sensitive K+Channels and L-type Ca2+ Channels in mouse pancreatic beta cell line MIN6.Methods MIN6 cells were divided into 0.05 ％ dimethyl sulfoxide (DMSO) group,normal control group and low-,middle-,high-concentration of Simvastatin treatment groups,that were cultured for 48 h with high-glucose DMEM containing 15％ fetal bovine serum plus 0.05％ dimethyl sulfoxide,0,2,5 and 10 μmol/Lsimvastatin,respectively.Whole-cell patch-clamp technology was used to record the currents of ATP-sensitive K+ channels and L-type Ca2+ channels in MIN6 cells.Results The mean potassium current density in normal external solution perfusion group was (92.81 ±4.10) pA/pF.Compared with normal external solution perfusion control group,2,5 and 10 μmol/L Simvastatin treatments markedly enhanced the current density of ATP-sensitive K+ Channels,reaching to (117.94 ± 3.67)pA/pF,(153.91±12.38) pA/pF,(307.01±6.40) pA/pF (all P＜0.01),respectively.The current density in L-type Ca2+ Channels was (-21.03 ± 0.55) pA/pF in glucose external solution group.Compared with glucose external solution group,the current density in 2,5 and 10 μmol/L Simvastatin treatment groups were decreased to (16.31±0.51) pA/pF,(-10.75±0.71) pA/pF,(-3.30±0.46) pA/pF (all P＜0.01),respectively.Conclusions Simvastatin inhibits insulin secretion and glycometabolism in mouse pancreatic beta cell line MIN6 via enhancing the current density of ATP-sensitive K+ Channels and inhibiting the current density of L-type Ca2+ Channels.
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Objective: To establish a simple, stable and high success rate separation method of myocardial cells in New Zealand rabbits. Methods: Compared influence between perfusion speed of 50 r/min (8 ml/min) and 65 r/min (11 ml/min) of Langendorff perfusion device on cell separation during separation course of myocardial cells. Results: Compared with perfusion speed of 50 r/min, there was significant decrease in time to obtain cells [(33±9.10) min vs. (19.29±2.73) min], and significant increase in success rate (50% vs. 91%) and survival rate [(38.26±2.63) % vs. (85.71±2.21) %] by perfusion speed of 65 r/min, P<0.01 all. Conclusion: Perfusion speed is an important factor that affects acute separation of myocardial cells in New Zealand rabbits, and 65r/min perfusion speed may be more stable and successful to obtain single myocardial cell.
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Objective To evaluate the importance of plasma MicroRNAs in early diagnosis of acute myocardial infarction (AMI).Methods 24 patients with AMI as the test group and 20healthy volunteers as the control group were enrolled in this study.Plasma levels of microRNA-1,microRNA-133a,microRNA-208a and microRNA-499 were detected by quantitative real time polymerase chain reaction (qRT-PCR) at 3 h,6 h,12 h,24 h,48 h,72 h after the onset of AMI.Results Plasma microRNA-1 level was greatly increased and reached the peak at 3 h after AMI,then was decreased gradually to normal level at 72 h after AMI.Plasma microRNA-133a level was significantly elevated at 6 h after AMI,reached peak at 12 h after AMI,then was decreased to normal level at 48 h after AMI.Plasma microRNA-1 and microRNA-133a levels were correlated with cTnI expression.The peak time of microRNA-1 was earlier than that of cTnI,while the peak time of microRNA-133a was the same as that of cTnI.Conclusions Increased circulating microRNA-1 and microRNA-133a may serve as potential and novel biomarkers for early diagnosis of AMI.
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Objective To investigate the value of plasma miR-22 in diagnosis of idiopathic pulmonary arterial hypertension ( IPAH ),and its role of regulation mechanisms in the pathogenesis of the disease.Methods Circulating miR-22 levels of IPAH patients and healthy controls were evaluated by RTPCR.The silico analysis of targets for miR-22 was taken, and followed by eGFP reporter assay for verification of predicted target gene Myc binding protein (MYCBP). Results Compared with healthy controls,the expression of plasma miR-22 in IPAH patients was significantly decreased (P ＜ 0.01 ).The area under curve (AUC) of ROC curve was 0.744.MYCBP was a real target of miR-22 confirmed by silico analysis and eGFP reporter assay. Conclusions The expression of plasma miR-22 was significantly decreased in IPAH patients,and it could serve as a potential biomarker for diagnosis.The miR-22 might be involved in the pathogenesis of the disease through promoting its target gene MYCBP to activate the c-Myc pathway.
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Objective To explore the predictive value of ventricle late potential (LP) for arrhythmic events in the patients with Brugada syndrome. Methods Totally 43 patients with Brugada syndrome were divided into symptom group (n=24) and asymptom group (n=19).Signalaveraged electrocardiography(SAECG) was performed to analyze characteristics of LP in all subjects.The occurrence of arrhythmic events was observed in all patients during the dynamic follow-up for (33.8±9.0) months.Results There were 22 cases (91.7％) and 7 cases (36.8％) with LP positive in patients with symptom and asymptom,respectively.The incidences of arrhythmic events were 72.4％ in Brugada syndrome patients with positive LP and 14.3％ in patients with negative LP,respectively.The relative risk (RR,95％ CI) for LP prediction of the arrhythmic events was [5.1,(1.4～ 18.6)] (P =0.002). ConclusionsLP may be one of effective factors predicting arrhythmic events in the patients with Brugada syndrome.